Reviven “virus gigante” previamente desconocido que permaneció enterrado por más de 30,000 años.
Resumen: Investigadores franceses descubren lo que se cree que es un nuevo organismo del grupo conocido como “virus gigantes”, que permaneció enterrado en las profundidades del ‘permafrost’ siberiano por más de 30,000 años. A diferencia de otros virus, los “virus gigantes” pueden observarse utilizando microscopía de luz y sus genomas codifican más proteínas que la mayor parte de las bacterias o parásitos intracelulares eucarióticos. El nuevo microorganismo ha sido llamado Pithovirus sibericum. El descubrimiento de los doctores Jean-Michel Claverie y Chantal Abergel de la Universidad Aix-Marseille en France, se detalla en la revista Proceedings of the National Academy of Sciences.
Summary: French researchers have discovered what is thought to be the newest representative of what are known as “giant viruses”, that remained buried deep in the Siberian permafrost and untouched for over 30,000 years. In contrast to other viruses, giant DNA viruses are visible under a light microscope and their genomes encode more proteins than some bacteria or intracellular parasitic eukaryotes. The newly discovered virus has been named Pithovirus sibericum. The findings of Jean-Michel Claverie and Chantal Abergel of the Aix-Marseille University in France, are detailed in the Proceedings of the National Academy of Sciences.
Lest Siberian excavation makes you think of frozen woolly mammoths, make no mistake: These giants are still decidedly microscopic. But in the diminutive world of viruses they’re larger than normal specimens, measuring 1.5 microns in length and 0.5 microns in diameter. The pandoraviruses, the largest viruses previously discovered, also by the team of Claverie and Abergel, measure 1 micron in length and 0.5 in diameter. “‘Giant’ viruses are loosely defined as the ones that you can see under a regular microscope,” explained Claverie and Abergel when contacted via email.
Giant viruses also dwarf other viruses in terms of genetic complexity. The newly discovered Pithovirus contains 500 genes, and the aforementioned Pandoravirus can contain up to 2,500. For comparison, the HIV virus contains only about 12 genes, explained James Van Etten, a professor of plant pathology at the University of Nebraska, when reached for comment. (Van Etten is an authority on viruses and edited the new study.) Amazingly, even after more than 30,000 years embedded in ancient permafrost, when Claverie and Abergel exposed amoebas in their lab to the virus, they found that the virus was still active and quickly infected the host cell. “We use amoeba on purpose as a safe bait for capturing viruses. We then immediately verify that they are not able to infect animal/human cells,” stressed the researchers.
Giant viruses are not just bigger but are hardier than others as well, said the researchers. This hardiness, along with a favorable environment, likely helped the newly discovered specimen stay intact for the thousands of years that it did. Viruses are often destroyed or rendered inactive by a number of factors, including light and biochemical degradation. “Among known viruses, the giant viruses tend to be very tough, almost impossible to break open,” said Claverie and Abergel. “Special environments such as deep ocean sediments and permafrost are very good preservers of microbes [and viruses] because they are cold, anoxic [lacking oxygen], and in the dark.”
The past decade has seen something of a renaissance in the discovery of large, genetically complex viruses, with the discovery of three distinct groups (Mimivirus, Pandoravirus, and now Pithovirus) suggesting that viruses can be much more intricate and varied than previously thought, and that giant viruses may not be especially uncommon. The newly discovered diversity in genetic makeup and morphology among viruses leads Van Etten to surmise that different types of viruses may have evolved separately. “The idea that all viruses evolved from one common origin, I suspect is not true,” said Van Etten.
If long-buried viruses can be unearthed, what else might be capable of coming to the surface? Climate change as well as industrial activities may shake up the ancient ice enough to bring potential pathogens to the surface. “Mining and drilling means … digging through these ancient layers for the first time in millions of years. If ‘viable’ [viral particles] are still there, this is a good recipe for disaster,” said Claverie and Abergel.
But Edward Mocarski, a professor of microbiology at Emory University, says the risk of a virus pathogenic to humans being released from the ice is very small. “The idea would make a great movie but is extremely unlikely unless the virus came from a frozen human being who possibly died from a virus that is no longer in circulation,” said Mocarski when contacted via email. “A very small proportion [of the viruses on Earth] represent viruses that can infect mammals and an even smaller proportion pose any risk to humans.”
University of Nebraska’s Van Etten agreed that such a situation was unlikely but possible with the right conditions. “The biggest source of genes on the planet is probably from viruses, and they’re just everywhere, but in general they’re highly specific for the organisms that they grow in,” said Van Etten. The researchers behind the discovery, Claverie and Abergel, believe that whether or not it’s likely, such a scenario remains feasible. They counsel vigilance and continued testing. As their latest research has shown, large DNA viruses may remain infectious for very long stretches of time.
“The fact that we might catch a viral infection from a long-extinct Neanderthal individual is a good demonstration that the notion that a virus could be ‘eradicated’ from the planet is plain wrong and gives us a false sense of security. At least a stock of vaccine should be kept, just in case,” said Claverie and Abergel. Their research will now turn to assessing how real a threat these ancient viruses pose.
“We are now doing more work to analyze the DNA content of these permafrost layers in a search for the genetic signature of viruses resembling human pathogens,” said Claverie and Abergel, who stressed that they are not attempting to “revive” any such pathogenic viruses, but rather hoping to determine the potential danger. “If we find some [human pathogens], then the risk will become more real. If not, we will be safe.”
Journal reference: Legendre M, Bartoli J, Shmakova L, Jeudy S, Labadie K, Adrait A, Lescot M, Poirot O, Bertaux L, Bruley C, Couté Y, Rivkina E, Abergel C, Claverie J-M. Thirty-thousand-year-old distant relative of giant icosahedral DNA viruses with a pandoravirus morphology. PNAS 2014; doi:10.1073/pnas.1320670111
Journal abstract: The largest known DNA viruses infect Acanthamoeba and belong to two markedly different families. The Megaviridae exhibit pseudo-icosahedral virions up to 0.7 μm in diameter and adenine–thymine (AT)-rich genomes of up to 1.25 Mb encoding a thousand proteins. Like their Mimivirus prototype discovered 10 y ago, they entirely replicate within cytoplasmic virion factories. In contrast, the recently discovered Pandoraviruses exhibit larger amphora-shaped virions 1 μm in length and guanine–cytosine-rich genomes up to 2.8 Mb long encoding up to 2,500 proteins. Their replication involves the host nucleus. Whereas the Megaviridae share some general features with the previously described icosahedral large DNA viruses, the Pandoraviruses appear unrelated to them. Here we report the discovery of a third type of giant virus combining an even larger pandoravirus-like particle 1.5 μm in length with a surprisingly smaller 600 kb AT-rich genome, a gene content more similar to Iridoviruses and Marseillevirus, and a fully cytoplasmic replication reminiscent of the Megaviridae. This suggests that pandoravirus-like particles may be associated with a variety of virus families more diverse than previously envisioned. This giant virus, named Pithovirus sibericum, was isolated from a >30,000-y-old radiocarbon-dated sample when we initiated a survey of the virome of Siberian permafrost. The revival of such an ancestral amoeba-infecting virus used as a safe indicator of the possible presence of pathogenic DNA viruses, suggests that the thawing of permafrost either from global warming or industrial exploitation of circumpolar regions might not be exempt from future threats to human or animal health.